Lipidure® PMD - All rights reserved

Polyquaternium-51

  • Prevents biofilm formation
  • Reduces plaque formation significantly
  • Reduces halitosis

Protects against primary infection

Protocol

  • HAD (hydroxyapatite disk) is treated with filtered human whole saliva for 10 min, and with Lipidure® PMD (commercial solution - pure) or destilled water for 10 min at room temperature. Streptococcal suspension (1.0 x 1.07 CFU/mL) is added and incubated at 37°C for 1 and 5 hours. After incubation, adherent cells are counted by image analysis of 10 randomly selected highpower fields by SEM, and the number of bacteria/1mm is calculated.

Results

Lipidure® PMB significantly inhibits the adhesion of Streptococcus mutans to hydroxyapatite plate by 84% at one hour and 94% at 5 hours.

Prevents biofilm formation of Streptococcus mutans and HAD - In Vitro

Protocol

  • HAD is treated with filtered human whole saliva for 10 min, and with Lipidure® PMB (commercial solution - pure) or distilled water for 10 min at room temperature. Streptococcal suspension ( 1.0 x 1.0 7 CFU/mL) is added and incubated at 37°C for 1 and 5 hours. After incubation, optical density of the bacterial suspension collected from biofilm with 1.0 N NaOH is measured at 655mm. Percent biofilm formation (control=100%) is expressed.

Results

Treatment with Lipidure® PMB prevents biofilm formation even after 30 seconds.

Protects the mouth over time - In Vitro

Protocol

  • Biofilms of S.mutans or S.intermedius formed on a hydroxyapatite film are coated with filtered human whole saliva for 10 min at room temperature, and with Lipidure® PMB (commercial solution - pure) or PBS for 30 sec. After washing the discs with PBS, F.nucleatum is added to streptococcal biofilms and anaerobically cultured at 37°C for 1 hour. After cultivation, the adhesion of F.nucleatum to the streptococcal biofilm are observed by SEM.

Results

Lipidure® PMB significantly reduces the dental plaque formation. Lipidure® PMB treatment significantly inhibits the adherence of F.nucleatum to both saliva-treated streptococcal biofilms in 30 seconds.

Mouth wash decreases halitosis

Protocol

  • 9 volunteers use mouth wash with or without Lipidure® PMB just before bed during 3 days. Halitosis is measured just after waking up with an Oralchroma, a simple gas chromatography system which measures 3 major halitosis substances:
  • Hydrogen sulfide (coated tongue)
  • Methyl mercaptan (plaque)
  • Dimethyl sulfide (lung)

Results

Lipidure® PMB mouth wash suppresses halitors.

Mouth wash helps maintain oral moisturization

Protocol

  • 3 volunteers wash their mouth with:
  • 3% Lipidure® PMB formulated mouth wash
  • Commercial mouth wash 1
  • Commercial mouth wash 2
  • Water
  • The moisture amount in oral cavity is measured after using the mouth wash with Oral cavity moisturizing meter MUCUS (Life Co.Ltd.)

Results

Lipidure® PMB formulated mouth wash can maintain the moisture content in the oral cavity.

Ectoin

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Betaïne

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Polyquaternium-51

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